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Literature summary extracted from

  • Larsen, A.N.; Moe, E.; Helland, R.; Gjellesvik, D.R.; Willassen, N.P.
    Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp (2006), FEBS J., 273, 47-60.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.64
-
 Parengyodontium album
3.4.21.64
-
 Serratia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.64 EDTA 22°C, or 37°C, not inhibitory, 50°C, 50% inhibition Parengyodontium album
3.4.21.64 EDTA 22°C, not inhibitory, 37%, 60% residual activity, 50°C, complete inhibition Serratia sp.
3.4.21.64 additional information not inhibitory: sodium dodecylsulfate Parengyodontium album
3.4.21.64 additional information not inhibitory: sodium dodecylsulfate Serratia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.64 0.46
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 22°C Parengyodontium album
3.4.21.64 0.48
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 12°C Parengyodontium album
3.4.21.64 0.52
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 37°C Parengyodontium album
3.4.21.64 2.36
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 12°C Serratia sp.
3.4.21.64 2.46
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 22°C Serratia sp.
3.4.21.64 2.76
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 37°C Serratia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.64 34000
-
 x * 65500, precursor-protein, calculated, x * 34000, mature protein, SDS-PAGE Serratia sp.
3.4.21.64 65500
-
 x * 65500, precursor-protein, calculated, x * 34000, mature protein, SDS-PAGE Serratia sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.64 Parengyodontium album
-
-
-
3.4.21.64 Serratia sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.64 proteolytic modification sequence consists of preproregion, catalytic domain and two C-terminal domains. Catalytic domain with the two C-terminal domains is an active protein of 56 kDa that converts at 50°C by autolytic cleavage to mature 34 kDa protein Serratia sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.64 88.6
-
-
 Parengyodontium album
3.4.21.64 88.6
-
-
 Serratia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.64 succinyl-AAPF-4-nitroanilide + H2O
-
 Parengyodontium album ?
-
 ?
3.4.21.64 succinyl-AAPF-4-nitroanilide + H2O
-
 Serratia sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.64 ? x * 65500, precursor-protein, calculated, x * 34000, mature protein, SDS-PAGE Serratia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.64 70
-
-
 Serratia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.64 37
-
 30 min, about 90% residual activity even in presence of 1% sodium dodecylsulfate Serratia sp.
3.4.21.64 50
-
 30 min, 19% residual activity in presence of 1% sodium dodecylsulfate Parengyodontium album
3.4.21.64 50
-
 30 min, 90% residual activity even in presence of 1% sodium dodecylsulfate Serratia sp.
3.4.21.64 70
-
 half-life 30 min Parengyodontium album
3.4.21.64 70
-
 half-life 19 min Serratia sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.64 51
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 12°C Parengyodontium album
3.4.21.64 88
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 22°C Parengyodontium album
3.4.21.64 175
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 12°C Serratia sp.
3.4.21.64 180
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 37°C Parengyodontium album
3.4.21.64 364
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 22°C Serratia sp.
3.4.21.64 827
-
 succinyl-AAPF-4-nitroanilide pH 8.0, 37°C Serratia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.64 8
-
-
 Parengyodontium album
3.4.21.64 10.5
-
-
 Serratia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.64 8 9.5
-
 Parengyodontium album
3.4.21.64 8 11
-
 Serratia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.64 4 12
-
 Parengyodontium album
3.4.21.64 5.5 9.5
-
 Serratia sp.